Metkinen Universal Solid Support III
The universal support strategy offers the following clear advantages: eliminates the possibility of errors in parallel synthesis applications where up to 384 wells may contain different supports; eliminates the need for at least four supports for DNA synthesis and four for RNA synthesis; simplifies the preparation of oligonucleotides with modified or unusual nucleosides at the 3-terminus.
Like our initial version of Universal Solid supports - USII, the new type of supports - Metkinen Universal Solid Support III would be appropriate for the production of DNA oligos, long and short, as well as those requiring mild deprotection. It is compatible with the synthesis of RNA, (including siRNA) as well as virtually any oligonucleotide analogs (2-F-RNA, 2-OMe-RNA, LNA, oligonucleotide
phosphoramidates, etc.). The reagent used for the cleavage/dephosphorylation step is commercially available and the procedures described are fully compatible with high-throughput Synthesis.
The difference lies in the higher stability of Metkinen Universal Solid Support III than that of USII upon prolong storage. In addition, the preparation of the new support appears to be more consistent and reliable.
Metkinen Universal Solid Support III is subject to proprietary rights of Glen Research Corporation and is synthesized and sold under the following licensed patents: US Patent No.: 6,770,754 and European Patent No.: 1404695. The new carbomoylation chemistry, resulting in the stable urea fragment, bridging the Universal linker and aminoalkylated solid phase, is subject to proprietary rights of Metkinen Chemistry (U.S. Patent Application Serial No. 60/854,721; International Patent Application No. PCT/ FI2007/050575).
Metkinen Universal Solid Support III for synthesis of DNA, RNA & any type of modified oligonucleotides.
Catalogue number: 103-00
Description: Chemically modified Macropourous
Aminomethyl polystyrene. White to
Storage of dry compound: 1 year at +20ēC
Loading: 20-80 µmol/g g loadings is available.
Please enquire for custom loading.
Oligo synthesis on USIII: Perform oligonucleotide
assembly, using standard protocols, recommended
by your synthesizer manufacturer. Upon
the completion of synthesis wash the oligonucleotide
bound support with pure acetonitrile. Do
not perform any washing steps with solvents,
containing basic reagents (diethylamine, triethylamine,
dimethylamine, etc.) and water!
Cleavage: Cleave the oligo from the support
using 3.5N - 4.5N ammonia in methanol (dilute
cold 7N ammonia in methanol, Aldrich Cat. No
499145-100ML, with cold anhydrous methanol)
at room temperature for 30 minutes. Do
not use aqueous ammonium hydroxide and/or
mixtures of ammonium hydroxide and methanol
Deprotection AFTER Cleavage:
Standard: After Cleavage, add 1 volume of 3.5N
- 4.5N ammonia in methanol, seal and deprotect
for 8-15 hours at 60 °C for removal of the
protecting groups on the nucleobases.
Alternatively: add 1 volume of 30% ammonium
hydroxide, seal and deprotect using the conditions
appropriate for removal of the protecting
groups on the nucleobases (e.g. at 55 °C for 5 hours).
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