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Metkinen Chemistry focuses on research and manufacture of reagents for RNA and DNA synthesis. We have expert knowledge of nucleoside chemistry, reagents for oligonucleotide synthesis, phosphoramidites and various solid supports. Reagents for oligo synthesis product line include Truly Universal solid support, Chemical Phosphorylation Reagent and Trimer (codon) Phosphoramidites. Possible applications encompass combinatorial oligonucletide libraries with trimer (codon) phosphoramidites, oligo synthesis using our Truly Universal solid support and convenient purification of oligonucleotide 5'-phosphates with Chemical Phosphorylation Reagent. Today the efforts of Metkinen Chemistry are concentrated on our extensive line of purine nucleosides. Ultra-pure purine nucleoside analogs are used in the development of oligonucleotide therapeutics and in research applications. The new products, namely 3'- Amino-2',3'-dideoxy-A and G nucleosides serve as building blocks for oligonucleotides that are resistant to enzymatic degradation. Another set of new nucleosides - 2'-deoxy-2'-fluoro-A and G, as well as 2'-deoxy-2'-amino-A and G are popular building clocks for the stabilization of small interfering RNAs (siRNAs) and aptamers. Metkinen Chemistry's biotransformation technology addresses the problems of traditional synthetic methods and offers alternative variants of biotransformation. Our flexibility and expertise allow Metkinen Chemistry to cater to a range of customer needs: from one-off oligonucleotide synthesis in academia to large-scale commercial production. Naturally this is reflected in appropriate discounts applied according to order size.
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Universal Support III (USIII) is appropriate for the production of DNA and RNA and modified oligonucleotides from laboratory to industrial scale. It requires fast and mild cleavage conditions.
Chemical Phosphorylation Reagent allows convenient purification of oligonucleotide 5'-phosphates by the straightforward DMTr-on technique.
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Universal Solid Support III
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Chemical Phosphorylation Reagent II
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Purine 2'-Fluoro-2'deoxynucleosides are obtained via biotransformation. They are extra pure and efficiently prices.
2'-Fluoro-2'-deoxynucleoside-3'-CE Phosphoramidites. Oligonucleotides, incorporating 2'-F-nucleoside units are widely employed in RNA and aptamer research.
2'-Fluoro-2'-deoxynucleoside-5'-triphosphates are popular products for various applications:
PCR and DNA-, RNA-polymerase, reverse transcriptase based assays.
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2’-Fluoro-2’-Deoxyguanosine
2’-Fluoro-2’-Deoxyadenosine
2'-Fluoro-2'-deoxynucleoside-5'-triphosphates
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2’-Fluoro-2’-Deoxyguanosine-3’-CE-phosphoramidite
2’-Fluoro-2’-Deoxyadenosine-3’-CE-phosphoramidite
2’-Fluoro-2’-Deoxyuridine-3’-CE-phosphoramidite
2’-Fluoro-2’-Deoxycytidine-3’-CE-phosphoramidite
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Biotin-11-dUTP
6-FAM-11-dUTP
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Biotin-16-dUTP
Biotin-11-dATP
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Biotin-11-dCTP
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6-FAM-11-dCTP
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Biotin-11-UTP
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6-FAM-11-UTP
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5’-Fluorescein phosphoramidite (6-FAM)
5'-Fluorescein phosphoramidite (5-FAM)
Fluorescein phosphoramidite (I)
Fluorescein phosphoramidite (II)
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6-Fluorescein dT phosphoramidite
5-Fluorescein dT phosphoramidite
3'-(6-Fluorescein)-CPG
3'-(5-Fluorescein)-CPG
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HEX phosphoramidite
Internal HEX phosphoramidite
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3'-HEX-CPG
SIMA phosphoramidite
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TET phosphoramidite
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C6 TFA Aminolinker Phosphoramidite
C3 MMTr-Aminolinker Phosphoramidite
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DMT C6-Aminolinker Phosphoramidite
C6 MMTr-Aminolinker Phosphoramidite
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Spacer 18 Phosphoramidite
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- Visit Metkinen Chemistry at TIDES 2008, Booth 217, May 18-21, Red Rock Casino, Resort & Spa, Las Vegas, NV, www.ibclifesciences.com/tides
- 2'-Fluoro-2'-deoxynucleosides. In the talk titled "Translating RNAi," Dr. Manoharan (Alnylam) presented new data related to the effective delivery of RNAi therapeutics. Dr. Manoharan showed that among the numerous carbohydrate modifications to improve the drug-like properties of siRNAs, 2'-Fluoro modifications consistently provided increased target binding affinity, endonuclease stability, and, importantly, reduced immune response. In addition, it is the only modification compared to other carbohydrate modifications, including locked nucleic acid, or LNA, that preserves RISC activity when used in both sense and antisense strands. Data presented included the following:
2'-Fluoro modified siRNAs are significantly more stable, thermodynamically and to nucleases in biological fluids, and can increase the efficacy of siRNAs approximately 2-fold in vivo; and 2'-Fluoro modified siRNA targeting Factor VII was found to produce no immuno-stimulatory response, whereas a corresponding unmodified siRNA could stimulate IFN-alpha and TNF-alpha in vitro. Read
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Trinucleotide and Dinucleotide phosphoramidite building blocks are reagents of choice for generation of highly randomized DNA sequences. These sequences may incorporate
20 codons, corresponding to all natural amino acids.
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Trinucleotide (TRIMER CODON) Phosphoramidites
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Dimer Phosphoramidites
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3'-Amino-2',3' dideoxynucleosides are synthesized via biotransformation and used as initial compounds for preparation of corresponding 3'-Amino-2',3'-Dideoxynucleoside-5'-CE Phosphoramidites. The latter are building blocks for the assembly of Oligonucleotide N3' -> P5' phosphoramidates, that form extremely stable double and triple stranded complexes with DNA and RNA under physiological conditions and appear to be efficient telomerase inhibitors and antisense agents.
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3'-Amino-2',3'-dideoxyadenosine
3'-Amino-2',3'-dideoxyguanosine
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3'-Amino-2',3'-dideoxy-2-fluoroadenosine
3'-Amino-2',3'-dideoxy-2,6-diaminopurineriboside
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Analogues of Purine nucleosides have potential interest in antiviral research and are indispensable tools in Molecular biology. They are building blocks for the synthesis of pharmaceutical
oligonucleotide analogues and initial compounds for preparation of corresponding nucleoside 5'-di- and triphosphates.
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2’-Amino-2’-deoxyadenosine
2'-Amino-2'-deoxy-2-fluoroadenosine
2’-Amino-2’-deoxy-2,6-diaminopurineriboside
2’-Amino-2’-deoxyguanosine
2’-Amino-2’-deoxyinosine
Arabinofuranosyl-adenine (adenine arabinoside, ara-A)
Arabinofuranosyl-6-Benzylaminopurine (6-Benzylaminopurine arabinoside)
Arabinofuranosyl-2,6- diaminopurine (2,6-diaminopurine arabinoside)
Arabinofuranosyl-2-fluoroadenine
Arabinofuranosyl-guanine (guanine arabinoside, ara-G)
Arabinofuranosyl-isoguanine
6-Benzylaminopurine 2'-deoxyriboside
2-Chloro-2'-deoxyadenosine (2-CdA, cladribine)
2'-Deoxy-2-fluoroadenosine
2'-Deoxy-2'-fluoro-2-fluoroadenosine
2'-Deoxy-2'-fluoro-2,6-diaminopurineriboside
2'-Deoxy-2'-fluoroinosine
2'-Deoxyisoguanosine
2'-Deoxy-2'-fluoroisoguanosine
2,6-Diaminopurine 2'-deoxyriboside
Isoguanosine
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Pyrene Derivative
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Pyrelene Derivative
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Reagent 1-amidite
Reagent 3-amidite
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Reagent 2-amidite
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- 5'-triphosphates
In the year 2007 Metkinen Chemistry starts production of 5'-triphosphates of nucleosides, listed in this catalog. The main goal of launching of this product line is to offer scientist a broad range of Modified Nucleoside 5'-triphosphates at a very competitive price, read
- Metkinen Chemistry Applies Biotransformation Technology to Control and Improve Costs
Metkinen Chemistry unveiled a biotransformation technology that has enabled for the first time the cost-effective commercial production of purine nucleoside analogues for use in the development of oligonluceotide therapeutics and for research applications... read
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