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6-Fluorescein dT phosphoramidite5'-Dimethoxytrityl-5-[3-(6-(fluoresceinyl-6-carboxamido)hexanoylamido)-propenyl]uridine, 3'- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite Catalogue number: 303-45 Diluent: Anhydrous Acetonitrile Coupling: 10 minute coupling time recommended Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 7-10 days, <90% efficient after 14 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia.
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