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TET phosphoramidite[(4,7,2',7'-tetrachloro-3',6'-dipivaloylfluoresceinyl)-6-carboxamidohexyl]-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite Catalogue number: 303-61 Diluent: Anhydrous Acetonitrile Coupling: 3 minute coupling time recommended Deprotection: Ammonium Hydroxide for 24 hrs at room temperature. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 2-3 days, <90% efficient after 4 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia.
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