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HEX-dT phosphoramidite5'-Dimethoxytrityl-5-[3-(6-(4,7,2',4',5',7'-hexachloro-3',6'-dipivaloylfluoresceinyl-6-carboxamido)hexanoylamido)-propenyl]uridine, 3'-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite Catalogue number: 303-53 Diluent: Anhydrous Acetonitrile Coupling: 10 minute coupling time recommended Deprotection: Ammonium Hydroxide for 24 hrs at room temperature. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 7-10 days, <90% efficient after 14 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia.
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