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Cy5 phosphoramidite1-[3-(4-monomethoxytrityloxy)propyl]-1'-[3-[(2-cyanoethyl)-(N,N-diisopropylphosphoramidityl]propyl]-3,3,3',3'-tetramethylindodicarbocyanine chloride Catalogue number: 303-73 Diluent: Anhydrous Acetonitrile Coupling: 3 minute coupling time recommended. Monomers and Cap A Mix that allow for UltraMILD deprotection are preferred. Use 0.02M iodine to avoid degradation of the cyanine dye. Deprotection: If UltraMILD reagents were used, deprotect at room temperature in 0.05M Potassium Carbonate in Methanol for 4 hrs or Ammonium Hydroxide for 2 hrs. If standard bases were used, deprotection in Ammonium Hydroxide at room temperature for 24-36 hrs will give acceptable yields. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 1-2 days, <70% efficient after 2 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia. Price: 1g 2290 euro |
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