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Cy3.5 phosphoramidite1-[3-(4-monomethoxytrityloxy)propyl]-1'-[3-[(2-cyanoethyl)-(N,N-diisopropyl)phosphoramidityl]propyl]-3,3,3',3'-tetramethyl-4,5-benzindocarbocyanine chloride Catalogue number: 303-72 Diluent: Anhydrous Acetonitrile Coupling: 3 minute coupling time recommended. Use 0.02M Iodine to avoid degradation of the cyanine dye. Deprotection: If standard bases were used, deprotect in Ammonium Hydroxide for 2 hours at 65°C or 24-36 hours at room temperature. If Ac-dC was used in the synthesis one may deprotect in AMA for 10 minutes at 65°C. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 2-3 days, <90% efficient after 4 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia. Price: 1g 2790 euro |
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