 |
 |
 |
|
||||||||||||||
 |
||||||||||||||
|
5'-Fluorescein phosphoramidite (5-FAM)[(3',6'-dipivaloylfluoresceinyl)-5-carboxamidohexyl]-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite Catalogue number: 303-42 Diluent: Anhydrous Acetonitrile Coupling: 3 minute coupling time recommended Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Storage: Freezer storage, -10 to -30°C, dry. Stability in Solution: 2-3 days, <90% efficient after 4 days Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20°C. Do not store crude fluorescently labeled oligonucleotides in ammonia.
|
|
||||||||||||
