Metkinen Chemistry - reagents for DNA and RNA synthesis Metkinen Chemistry - Reagents for RNA and DNA synthesis.
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Metkinen Chemistry focuses on research and manufacture of reagents for RNA and DNA synthesis. We have expert knowledge of nucleoside chemistry, reagents for oligonucleotide synthesis, phosphoramidites and various solid supports. Reagents for oligo synthesis product line include Truly Universal solid support, Chemical Phosphorylation Reagent and Trimer (codon) Phosphoramidites. Possible applications encompass combinatorial oligonucletide libraries with trimer (codon) phosphoramidites, oligo synthesis using our Truly Universal solid support and convenient purification of oligonucleotide 5'-phosphates with Chemical Phosphorylation Reagent. Today the efforts of Metkinen Chemistry are concentrated on our extensive line of purine nucleosides. Ultra-pure purine nucleoside analogs are used in the development of oligonucleotide therapeutics and in research applications. The new products, namely 3'- Amino-2',3'-dideoxy-A and G nucleosides serve as building blocks for oligonucleotides that are resistant to enzymatic degradation. Another set of new nucleosides - 2'-deoxy-2'-fluoro-A and G, as well as 2'-deoxy-2'-amino-A and G are popular building clocks for the stabilization of small interfering RNAs (siRNAs) and aptamers. Metkinen Chemistry's biotransformation technology addresses the problems of traditional synthetic methods and offers alternative variants of biotransformation. Our flexibility and expertise allow Metkinen Chemistry to cater to a range of customer needs: from one-off oligonucleotide synthesis in academia to large-scale commercial production. Naturally this is reflected in appropriate discounts applied according to order size.

 2'-Deoxy-2'-fluoronucleosides & Derivatives
Synthetic RNA oligonucleotides, incorporating 2'-Deoxy-2-fluoro-nucleosides (2-F-RNA) find constantly increasing applications in research and new drug development. Among important areas of their application are such as design and synthesis of efficient and stable siRNAs, aptamers, anti-microRNAs (Antagomirs) as well as general use for affinity purification of RNA-binding proteins.

Today Metkinen Chemistry offers more than 99% pure purine 2-fluoronucleosides, prepared by bioconversion of synthetic 2-F-uridine, using evolved recombinant E.coli strains. These strains, in turn are grown on the media, incorporation only ingredients, derived from yeast and vegetable. That means that in the production process no secondary reagents of animal source (either BSE certified or non- BSE certified) are employed at any stage of 2-F- A and G preparation. Hence, we guarantee the complete absence of ANY BSE/TSE risk, associated with our 2-F-nucleosides and derivatives.

In addition to purine 2-F-nucleosides, we offer a full set of 2-F-nucleoside CE-phosphoramidites for chemical synthesis and a full set of 2-F-nucleoside 5-triphosphates for enzymatic preparation of 2-F-RNA. Our latest addition to series of 2-fluoronucleoside derivatives 2-F-nucleoside bound solid supports for 2-F-RNA oligosynthesis, complements the range of these compounds and opens up a full spectrum of 2-F-RNA applications. 2'-Fluoro modifications consistently provided increased target binding affinity, endonuclease stability, and, importantly, reduced immune response. In addition, it is the only modification compared to other carbohydrate modifications, including locked nucleic acid, or LNA, that preserves RISC activity when used in both sense and antisense strands.

  • 2-Deoxy-2-fluoroguanosine

  • 2-Deoxy-2-fluoroadenosine

  • 2-Deoxy-2-fluoroguanosine-3-CE-phosphoramidite

  • 2-Deoxy-2-fluoroadenosine-3-CE-phosphoramidite

  • 2-Deoxy-2-fluorouridine-3-CE-phosphoramidite

  • 2-Deoxy-2-fluorocytidine-3-CE-phosphoramidite


  •  2-Deoxy-2-fluoroarabinonucleosides (FANA) & Derivatives
    2-Fluoroarabinooligonucleotides (FANA) can be used as antisense oligonucleotides, siRNA and aptamers. Today Metkinen Chemistry offers synthetically synthesized pyrimidine 2-fluoroarabinonucleosides as well as purine 2- fluoroarabinonucleosides, prepared by bioconversion of synthetic 2-F-arabinouridine, employing propriarory evolved recombinant E.coli strains. We guarantee the complete absence of ANY BSE/TSE risk, associated with our 2-F- fluoroarabinonucleosides. Metkinen Chemistry offers a full set of 2-F-nucleoside CE-phosphoramidites for the chemical synthesis and a full set of 2-F-nucleoside 5-triphosphates for enzymatic preparation of FANA.

  • 9-(2-Deoxy-2-fluoro--D-arabinofuranosyl)adenine (2-FANA-A)

  • 9-(2-Deoxy-2-fluoro--D-arabinofuranosyl)-2,6-diaminopurine (FANA-DAP)

  • 9-(2-Deoxy-2-fluoro--D-arabinofuranosyl)guanine (2-FANA-G)

  • 9-(2-Deoxy-2-fluoro--D-arabinofuranosyl)hypoxantine (2-FANA-I)

  • 1-(2-Deoxy-2-fluoro--D-arabinofuranosyl)cytosine (2-FANA-C)

  • 1-(2-Deoxy-2-fluoro--D-arabinofuranosyl)uracil (2-FANA-U)

  • 9-(2-Deoxy-2-fluoro--D-arabinofuranosyl)-6-thioguanine (FANA-thioG)

  • 2'-Deoxy-2'-fluoroarabinoadenosine-3'-CE-phosphoramidite

  • 2'-Deoxy-2'-fluoroarabinoguanosine-3'-CE-phosphoramidite

  • 2'-Deoxy-2'-fluoroarabinouridine-3'-CE-phosphoramidite

  • 2'-Deoxy-2'-fluoroarabinocytidine-3'-CE-phosphoramidite


  •  Various Purine Nucleosides
    Analogues of Purine nucleosides have potential interest in antiviral research and are indispensable tools in Molecular biology. They are building blocks for the synthesis of pharmaceutical oligonucleotide analogues and initial compounds for preparation of corresponding nucleoside 5'-di- and triphosphates.

  • 2-Amino-2-deoxyadenosine

  • 2'-Amino-2'-deoxy-2-fluoroadenosine

  • 2-Amino-2-deoxy-2,6-diaminopurineriboside

  • 2-Amino-2-deoxyguanosine

  • 2-Amino-2-deoxyinosine

  • Arabinofuranosyl-adenine (adenine arabinoside, ara-A)

  • Arabinofuranosyl-6-Benzylaminopurine (6-Benzylaminopurine arabinoside)

  • Arabinofuranosyl-2,6-diaminopurine (2,6-diaminopurine arabinoside)

  • Arabinofuranosyl-2-fluoroadenine

  • Arabinofuranosyl-guanine (guanine arabinoside, ara-G)

  • Arabinofuranosyl-isoguanine

  • 6-Benzylaminopurine 2'-deoxyriboside

  • 2-Chloro-2'-deoxyadenosine (2-CdA, cladribine)

  • 2'-Deoxy-2-fluoroadenosine

  • 2-Deoxy-2-fluoro-2-fluoroadenosine

  • 2-Deoxy-2-fluoro-2,6-Diaminopurineriboside

  • 2-Deoxy-2-fluoroinosine

  • 2'-Deoxyisoguanosine

  • 2'-Deoxy-2'-fluoroisoguanosine

  • Isoguanosine


  •  SIMA Phosphoramidites and CPG
  • 6-SIMA phosphoramidite

  • 6-SIMA-dT phosphoramidite

  • 5-SIMA phosphoramidite

  • 6-SIMA-CPG


  •  FAM Phosphoramidites and CPG
  • 5-(6-Fluorescein) phosphoramidite (6-FAM)

  • 5'-(5-Fluorescein) phosphoramidite (5-FAM)

  • 6-Fluorescein dT phosphoramidite

  • FAM-CPG


  •  HEX Phosphoramidites
  • 6-HEX phosphoramidite


  •  TET Phosphoramidites
  • 6-TET phosphoramidite

  • 5-TET phosphoramidite


  •  Biotin Phosphoramidites
  • 5'-Biotin Phosphoramidite


  •  JOE phosphoramidites
  • 6-JOE phosphoramidite


  •  R6G Phosphoramidites
  • R6G phosphoramidite


  •  Dye Azides for Click and CATAPAZ Chemistries
  • 5-FAM-azide

  • 6-FAM-azide

  • 6-HEX-azide

  • 5-SIMA-azide

  • 6-SIMA-azide

  • 5-TET-azide

  • 6-TET-azide

  • 5-JOE-azide

  • 6-JOE-azide

  • Cyanine 3-azide

  • Cyanine 5-azide

  • Cyanine 3.5-azide

  • Cyanine 5.5-azide

  • Biotin-azide

  •  Reagents for RNA and DNA synthesis
    Metkinen Universal Support is appropriate for the production of DNA and RNA and modified oligonucleotides from laboratory to industrial scale. It requires fast and mild cleavage conditions. Chemical Phosphorylation Reagent allows convenient purification of oligonucleotide 5'-phosphates by the straightforward DMTr-on technique.

    Metkinen Chemistry uses proprietary technology for production of some reagents for DNA and RNA synthesis. The invention (CATAPAZ chemistry) relates to highly efficient and straightforward carbamoylation of amines, mercaptanes, thiophenols and some alcohols employing organic azides, triphenylphosphine or polymer bound triphenylphosphine along with aqueous hydrogen carbonate buffer.

    At present, the CATAPAZ chemistry is successfully used for the production of Universal solid support and new nucleoside bound supports for synthesis of DNA, RNA, siRNA as well as any type of modified oligonucleotides. CATAPAZ Patent No. US 8,779,194B2 has been published. Please follow the link to see patent description.

    Comprehensive coverage of CATAPAZ technology and application can be found in the following articles:
    1. Tetrahedron, Volume 66, Issue 12, 20 March 2010, Pages 2210-2221
    2. Nucleosides, Nucleotides and Nucleic Acids (2011), 30:7-8, 475-489

  • Metkinen Universal Solid Support III

  • Chemical Phosphorylation Reagent II

  • Trinucleotide (TRIMER CODON) Phosphoramidites

  • Alkyne Amidite, 5-terminal

  • 5-DMS(O)MT-Amino-Modifier-C6



  •  NEW! Trimer Phosphoramidites
    Trinucleotide phosphoramidite building blocks are reagents of choice for generation of highly randomized DNA sequences. These sequences may incorporate codons and anticodons, corresponding to all natural amino acids. Today all 25 Trimer (codon and anticodon) phosphoramidites are available from Metkinen Chemistry.

     NEW! RANDOMIZED OLIGONUCLEOTIDE LIBRARIES generated by using TRIMER PHOSPHORAMIDITES
    Metkinen Chemistry offers randomized oligonucleotide libraries synthesized by using synthetic Trinucleotide (codon and anticodon) phosphoramidites.

    Advantages of using Trinucleotide phosphoramidites for generation of randomized oligonucleotide libraries:
    • Absence of synonymous, rare and stop codons
    • Absence of frameshift mutations
    • Library variability can be more that an order higher than the ones obtained by common methods
    • Occurrence of each amino acid at a certain position can be easily controlled
    References:
    1. J. Mol. Biol. (2007) 373, 924940
    2. Protein Engineering, Design and Selection (2011) 24(9): 691-700;
    3. Protein Engineering, Design and Selection (2013) 26(10): 683-693.

    To order contact: info@metkinenchemistry.com


     Modified Nucleoside 5-Triphosphates
    Modified Nucleoside 5-triphosphates are popular products for various applications: PCR, DNA- and RNA-polymerase, reverse transcriptase based assays, etc. Nowadays these compounds find diverse use in siRNA and Aptamer research.

     2-Deoxy-2-fluoronucleoside 5-Triphosphates
  • 2-Deoxy-2-fluoroadenosine 5-Triphosphate

  • 2-Deoxy-2-fluorocytidine 5-Triphosphate

  • 2-Deoxy-2-fluoroguanosine 5-Triphosphate

  • 2-Deoxy-2-fluorouridine 5-Triphosphate

  • 2-Deoxy-2-fluoronucleoside-5-triphosphates Kit


  •  2-Deoxy-2-fluoroarabinonucleoside (FANA) 5-Triphosphates
  • 2-Deoxy-2-fluoroarabinoadenosine 5-triphosphate

  • 2-Deoxy-2-fluoroarabinoguanosine 5-triphosphate

  • 2-Deoxy-2-fluoroarabinouridine 5-triphosphate

  • 2-Deoxy-2-fluoroarabinocytidine 5-triphosphate

  • 2-Deoxy-2-fluoroarabinonucleoside 5-triphosphates Kit


  •  Modified Purine Nucleoside 5-Triphosphates
  • 2'-Deoxy-2,2'-difluoroadenosine 5-triphosphate, tetra-lithium salt

  • 2'-Deoxy-2-fluoroadenosine 5-triphosphate, tetra-lithium salt

  • 2-Fluoro-9--D-arabinofuranosyladenine 5-triphosphate, tetra-lithium salt

  • 2'-Deoxy-2-chloroadenosine 5-triphosphate, tetra-lithium salt

  • 6-Benzylamino-2'-deoxy-9--D-ribofuranosylpurine 5-triphosphate, tetra-lithium salt


  •  Biotin Labeled Triphosphates
  • Biotin-11-UTP

  • Biotin-11-dUTP

  • Biotin-11-dCTP

  • Biotin-11-dATP

  • Biotin-16-dUTP


  •  Fluorescein Labeled Triphosphates
  • 6-FAM-11-dUTP

  • 6-FAM-11-dCTP

  • 6-FAM-11-UTP

  • FAM-11-dATP


  •  Cyanine 3, Cyanine 5 Labeled Triphosphates
  • Cyanine 3-11-dUTP

  • Cyanine 5-11-dUTP

  • Cyanine 3-11-dCTP

  • Cyanine 5-11-dCTP

  • Cyanine 3-11-UTP

  • Cyanine 5-11-UTP


  •  Purine 3-Amino-2,3-Dideoxynucleosides & Derivatives
    3'-Amino-2',3' dideoxynucleosides are synthesized via biotransformation and used as initial compounds for preparation of corresponding 3'-Amino-2',3'-Dideoxynucleoside-5'-CE Phosphoramidites. The latter are building blocks for the assembly of Oligonucleotide N3' -> P5' phosphoramidates, that form extremely stable double and triple stranded complexes with DNA and RNA under physiological conditions and appear to be efficient telomerase inhibitors and antisense agents.

  • 3'-Amino-2',3'-dideoxyadenosine

  • 3'-Amino-2',3'-dideoxyguanosine

  • 3'-Amino-2',3'-dideoxy-2-fluoroadenosine

  • 3'-Amino-2',3'-dideoxy-2,6-diaminopurineriboside